Nonsecretor Histo–blood Group Antigen Phenotype Is Associated With Reduced Risk of Clinical Rotavirus Vaccine Failure in Malawian Infants

BACKGROUND: Histo–blood group antigen (HBGA) Lewis/secretor phenotypes predict genotype-specific susceptibility to rotavirus gastroenteritis (RVGE). We tested the hypothesis that nonsecretor/Lewis-negative phenotype leads to reduced vaccine take and lower clinical protection following vaccination wi...

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Autores principales: Pollock, Louisa, Bennett, Aisleen, Jere, Khuzwayo C, Dube, Queen, Mandolo, Jonathan, Bar-Zeev, Naor, Heyderman, Robert S, Cunliffe, Nigel A, Iturriza-Gomara, Miren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763638/
https://www.ncbi.nlm.nih.gov/pubmed/30561537
http://dx.doi.org/10.1093/cid/ciy1067
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author Pollock, Louisa
Bennett, Aisleen
Jere, Khuzwayo C
Dube, Queen
Mandolo, Jonathan
Bar-Zeev, Naor
Heyderman, Robert S
Cunliffe, Nigel A
Iturriza-Gomara, Miren
author_facet Pollock, Louisa
Bennett, Aisleen
Jere, Khuzwayo C
Dube, Queen
Mandolo, Jonathan
Bar-Zeev, Naor
Heyderman, Robert S
Cunliffe, Nigel A
Iturriza-Gomara, Miren
author_sort Pollock, Louisa
collection PubMed
description BACKGROUND: Histo–blood group antigen (HBGA) Lewis/secretor phenotypes predict genotype-specific susceptibility to rotavirus gastroenteritis (RVGE). We tested the hypothesis that nonsecretor/Lewis-negative phenotype leads to reduced vaccine take and lower clinical protection following vaccination with G1P[8] rotavirus vaccine (RV1) in Malawian infants METHODS: A cohort study recruited infants receiving RV1 at age 6 and 10 weeks. HBGA phenotype was determined by salivary enzyme-linked immunosorbent assay (ELISA). RV1 vaccine virus shedding was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in stool collected on alternate days for 10 days post-immunization. Plasma rotavirus–specific immunoglobulin A was determined by ELISA pre- and post-immunization. In a case-control study, HBGA phenotype distribution was compared between RV1-vaccinated infants with RVGE and 1:1 age-matched community controls. Rotavirus genotype was determined by RT-PCR. RESULTS: In 202 cohort participants, neither overall vaccine virus fecal shedding nor seroconversion differed by HBGA phenotype. In 238 case-control infants, nonsecretor phenotype was less common in infants with clinical vaccine failure (odds ratio [OR], 0.39; 95% confidence interval [CI], 0.20–0.75). Nonsecretor phenotype was less common in infants with P[8] RVGE (OR, 0.12; 95% CI, 0.03–0.50) and P[4] RVGE (OR, 0.17; 95% CI, 0.04–0.75). Lewis-negative phenotype was more common in infants with P[6] RVGE (OR, 3.2; 95% CI, 1.4–7.2). CONCLUSIONS: Nonsecretor phenotype was associated with reduced risk of rotavirus vaccine failure. There was no significant association between HBGA phenotype and vaccine take. These data refute the hypothesis that high prevalence of nonsecretor/Lewis-negative phenotypes contributes to lower rotavirus vaccine effectiveness in Malawi.
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spelling pubmed-67636382019-10-02 Nonsecretor Histo–blood Group Antigen Phenotype Is Associated With Reduced Risk of Clinical Rotavirus Vaccine Failure in Malawian Infants Pollock, Louisa Bennett, Aisleen Jere, Khuzwayo C Dube, Queen Mandolo, Jonathan Bar-Zeev, Naor Heyderman, Robert S Cunliffe, Nigel A Iturriza-Gomara, Miren Clin Infect Dis Articles and Commentaries BACKGROUND: Histo–blood group antigen (HBGA) Lewis/secretor phenotypes predict genotype-specific susceptibility to rotavirus gastroenteritis (RVGE). We tested the hypothesis that nonsecretor/Lewis-negative phenotype leads to reduced vaccine take and lower clinical protection following vaccination with G1P[8] rotavirus vaccine (RV1) in Malawian infants METHODS: A cohort study recruited infants receiving RV1 at age 6 and 10 weeks. HBGA phenotype was determined by salivary enzyme-linked immunosorbent assay (ELISA). RV1 vaccine virus shedding was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in stool collected on alternate days for 10 days post-immunization. Plasma rotavirus–specific immunoglobulin A was determined by ELISA pre- and post-immunization. In a case-control study, HBGA phenotype distribution was compared between RV1-vaccinated infants with RVGE and 1:1 age-matched community controls. Rotavirus genotype was determined by RT-PCR. RESULTS: In 202 cohort participants, neither overall vaccine virus fecal shedding nor seroconversion differed by HBGA phenotype. In 238 case-control infants, nonsecretor phenotype was less common in infants with clinical vaccine failure (odds ratio [OR], 0.39; 95% confidence interval [CI], 0.20–0.75). Nonsecretor phenotype was less common in infants with P[8] RVGE (OR, 0.12; 95% CI, 0.03–0.50) and P[4] RVGE (OR, 0.17; 95% CI, 0.04–0.75). Lewis-negative phenotype was more common in infants with P[6] RVGE (OR, 3.2; 95% CI, 1.4–7.2). CONCLUSIONS: Nonsecretor phenotype was associated with reduced risk of rotavirus vaccine failure. There was no significant association between HBGA phenotype and vaccine take. These data refute the hypothesis that high prevalence of nonsecretor/Lewis-negative phenotypes contributes to lower rotavirus vaccine effectiveness in Malawi. Oxford University Press 2019-10-15 2018-12-18 /pmc/articles/PMC6763638/ /pubmed/30561537 http://dx.doi.org/10.1093/cid/ciy1067 Text en © The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles and Commentaries
Pollock, Louisa
Bennett, Aisleen
Jere, Khuzwayo C
Dube, Queen
Mandolo, Jonathan
Bar-Zeev, Naor
Heyderman, Robert S
Cunliffe, Nigel A
Iturriza-Gomara, Miren
Nonsecretor Histo–blood Group Antigen Phenotype Is Associated With Reduced Risk of Clinical Rotavirus Vaccine Failure in Malawian Infants
title Nonsecretor Histo–blood Group Antigen Phenotype Is Associated With Reduced Risk of Clinical Rotavirus Vaccine Failure in Malawian Infants
title_full Nonsecretor Histo–blood Group Antigen Phenotype Is Associated With Reduced Risk of Clinical Rotavirus Vaccine Failure in Malawian Infants
title_fullStr Nonsecretor Histo–blood Group Antigen Phenotype Is Associated With Reduced Risk of Clinical Rotavirus Vaccine Failure in Malawian Infants
title_full_unstemmed Nonsecretor Histo–blood Group Antigen Phenotype Is Associated With Reduced Risk of Clinical Rotavirus Vaccine Failure in Malawian Infants
title_short Nonsecretor Histo–blood Group Antigen Phenotype Is Associated With Reduced Risk of Clinical Rotavirus Vaccine Failure in Malawian Infants
title_sort nonsecretor histo–blood group antigen phenotype is associated with reduced risk of clinical rotavirus vaccine failure in malawian infants
topic Articles and Commentaries
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763638/
https://www.ncbi.nlm.nih.gov/pubmed/30561537
http://dx.doi.org/10.1093/cid/ciy1067
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