Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis

BACKGROUND: Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in multiplex genome editing technologies. However, simultaneously modulating multiple genes on the chromosome remains challengin...

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Detalles Bibliográficos
Autores principales: Liu, Dingyu, Huang, Can, Guo, Jiaxin, Zhang, Peiji, Chen, Tao, Wang, Zhiwen, Zhao, Xueming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764132/
https://www.ncbi.nlm.nih.gov/pubmed/31572493
http://dx.doi.org/10.1186/s13068-019-1537-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764132/
https://www.ncbi.nlm.nih.gov/pubmed/31572493
http://dx.doi.org/10.1186/s13068-019-1537-1

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