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Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis
BACKGROUND: Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in multiplex genome editing technologies. However, simultaneously modulating multiple genes on the chromosome remains challengin...
Autores principales: | Liu, Dingyu, Huang, Can, Guo, Jiaxin, Zhang, Peiji, Chen, Tao, Wang, Zhiwen, Zhao, Xueming |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764132/ https://www.ncbi.nlm.nih.gov/pubmed/31572493 http://dx.doi.org/10.1186/s13068-019-1537-1 |
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