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Inhibition of polymerase chain reaction: Pathogen-specific controls are better than human gene amplification

PCR inhibition is frequent in medical microbiology routine practice and may lead to false-negative results; however there is no consensus on how to detect it. Pathogen-specific and human gene amplifications are widely used to detect PCR inhibition. We aimed at comparing the value of PCR inhibitor de...

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Detalles Bibliográficos
Autores principales: Roux, Guillaume, Ravel, Christophe, Varlet-Marie, Emmanuelle, Jendrowiak, Rachel, Bastien, Patrick, Sterkers, Yvon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764677/
https://www.ncbi.nlm.nih.gov/pubmed/31560697
http://dx.doi.org/10.1371/journal.pone.0219276