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High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution

Targeted PCR amplification and high-throughput sequencing (amplicon sequencing) of 16S rRNA gene fragments is widely used to profile microbial communities. New long-read sequencing technologies can sequence the entire 16S rRNA gene, but higher error rates have limited their attractiveness when accur...

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Detalles Bibliográficos
Autores principales: Callahan, Benjamin J, Wong, Joan, Heiner, Cheryl, Oh, Steve, Theriot, Casey M, Gulati, Ajay S, McGill, Sarah K, Dougherty, Michael K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765137/
https://www.ncbi.nlm.nih.gov/pubmed/31269198
http://dx.doi.org/10.1093/nar/gkz569