Joint profiling of DNA methylation and chromatin architecture in single cells

We report a method, Methyl-HiC, which captures the chromosome conformation and DNA methylome together in a cell. We use it to reveal coordinated DNA methylation status between distal genomic segments that are in spatial proximity in the nucleus and delineate heterogeneity of both the chromatin archi...

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Detalles Bibliográficos
Autores principales: Li, Guoqiang, Liu, Yaping, Zhang, Yanxiao, Kubo, Naoki, Yu, Miao, Fang, Rongxin, Kellis, Manolis, Ren, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765429/
https://www.ncbi.nlm.nih.gov/pubmed/31384045
http://dx.doi.org/10.1038/s41592-019-0502-z
Descripción
Sumario:We report a method, Methyl-HiC, which captures the chromosome conformation and DNA methylome together in a cell. We use it to reveal coordinated DNA methylation status between distal genomic segments that are in spatial proximity in the nucleus and delineate heterogeneity of both the chromatin architecture and DNA methylome in a mixed population. Methyl-HiC enables simultaneous characterization of cell-type specific chromatin organization and epigenome in complex tissues.

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