Joint profiling of DNA methylation and chromatin architecture in single cells

We report a method, Methyl-HiC, which captures the chromosome conformation and DNA methylome together in a cell. We use it to reveal coordinated DNA methylation status between distal genomic segments that are in spatial proximity in the nucleus and delineate heterogeneity of both the chromatin archi...

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Detalles Bibliográficos
Autores principales: Li, Guoqiang, Liu, Yaping, Zhang, Yanxiao, Kubo, Naoki, Yu, Miao, Fang, Rongxin, Kellis, Manolis, Ren, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765429/
https://www.ncbi.nlm.nih.gov/pubmed/31384045
http://dx.doi.org/10.1038/s41592-019-0502-z
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author Li, Guoqiang
Liu, Yaping
Zhang, Yanxiao
Kubo, Naoki
Yu, Miao
Fang, Rongxin
Kellis, Manolis
Ren, Bing
author_facet Li, Guoqiang
Liu, Yaping
Zhang, Yanxiao
Kubo, Naoki
Yu, Miao
Fang, Rongxin
Kellis, Manolis
Ren, Bing
author_sort Li, Guoqiang
collection PubMed
description We report a method, Methyl-HiC, which captures the chromosome conformation and DNA methylome together in a cell. We use it to reveal coordinated DNA methylation status between distal genomic segments that are in spatial proximity in the nucleus and delineate heterogeneity of both the chromatin architecture and DNA methylome in a mixed population. Methyl-HiC enables simultaneous characterization of cell-type specific chromatin organization and epigenome in complex tissues.
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spelling pubmed-67654292020-02-05 Joint profiling of DNA methylation and chromatin architecture in single cells Li, Guoqiang Liu, Yaping Zhang, Yanxiao Kubo, Naoki Yu, Miao Fang, Rongxin Kellis, Manolis Ren, Bing Nat Methods Article We report a method, Methyl-HiC, which captures the chromosome conformation and DNA methylome together in a cell. We use it to reveal coordinated DNA methylation status between distal genomic segments that are in spatial proximity in the nucleus and delineate heterogeneity of both the chromatin architecture and DNA methylome in a mixed population. Methyl-HiC enables simultaneous characterization of cell-type specific chromatin organization and epigenome in complex tissues. 2019-08-05 2019-10 /pmc/articles/PMC6765429/ /pubmed/31384045 http://dx.doi.org/10.1038/s41592-019-0502-z Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Li, Guoqiang
Liu, Yaping
Zhang, Yanxiao
Kubo, Naoki
Yu, Miao
Fang, Rongxin
Kellis, Manolis
Ren, Bing
Joint profiling of DNA methylation and chromatin architecture in single cells
title Joint profiling of DNA methylation and chromatin architecture in single cells
title_full Joint profiling of DNA methylation and chromatin architecture in single cells
title_fullStr Joint profiling of DNA methylation and chromatin architecture in single cells
title_full_unstemmed Joint profiling of DNA methylation and chromatin architecture in single cells
title_short Joint profiling of DNA methylation and chromatin architecture in single cells
title_sort joint profiling of dna methylation and chromatin architecture in single cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765429/
https://www.ncbi.nlm.nih.gov/pubmed/31384045
http://dx.doi.org/10.1038/s41592-019-0502-z
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