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New Functional Ingredients Based on Microencapsulation of Aqueous Anthocyanin-Rich Extracts Derived from Black Rice (Oryza sativa L.)

The aqueous anthocyanin-rich extract derived from black rice (Oryza sativa L.) was encapsulated by freeze drying using milk proteins and peptides as coating materials. The molecular modelling approach indicated that all major casein fractions and whey proteins were able to bind at least one anthocya...

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Autores principales: Aprodu, Iuliana, Milea, Ștefania Adelina, Anghel, Roxana-Mădălina, Enachi, Elena, Barbu, Vasilica, Crăciunescu, Oana, Râpeanu, Gabriela, Bahrim, Gabriela Elena, Oancea, Anca, Stănciuc, Nicoleta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766832/
https://www.ncbi.nlm.nih.gov/pubmed/31540422
http://dx.doi.org/10.3390/molecules24183389
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author Aprodu, Iuliana
Milea, Ștefania Adelina
Anghel, Roxana-Mădălina
Enachi, Elena
Barbu, Vasilica
Crăciunescu, Oana
Râpeanu, Gabriela
Bahrim, Gabriela Elena
Oancea, Anca
Stănciuc, Nicoleta
author_facet Aprodu, Iuliana
Milea, Ștefania Adelina
Anghel, Roxana-Mădălina
Enachi, Elena
Barbu, Vasilica
Crăciunescu, Oana
Râpeanu, Gabriela
Bahrim, Gabriela Elena
Oancea, Anca
Stănciuc, Nicoleta
author_sort Aprodu, Iuliana
collection PubMed
description The aqueous anthocyanin-rich extract derived from black rice (Oryza sativa L.) was encapsulated by freeze drying using milk proteins and peptides as coating materials. The molecular modelling approach indicated that all major casein fractions and whey proteins were able to bind at least one anthocyanin molecule. The hydrophobic interactions and hydrogen bonding across the interfaces appeared to be mainly responsible for the stabilizations of the complexes formed between the coating material and bioactive compounds. Two dark purple colored powders, differentiated by the ratio of the encapsulation materials used, rich in phytochemicals were obtained, with an encapsulation efficiency of up to 99%. The powders were tested for antioxidant activity, cytocompatibility, and thermal stability. The morphological structure of the powders highlighted the presence of encapsulated anthocyanins. Both powders showed a remarkable antioxidant activity of about 46 mM Trolox/g D.W., and cytocompatibility on the L929 fibroblast culture. At certain concentrations, both powders stimulated cell proliferation. The powders showed a good thermal stability between 75 and 100 °C for 15 min. The powders were tested in a food model system and checked for stability of phytochemicals during storage. The added value of the powders was demonstrated throughout the antioxidant activity, which remained unchanged during storage.
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spelling pubmed-67668322019-10-02 New Functional Ingredients Based on Microencapsulation of Aqueous Anthocyanin-Rich Extracts Derived from Black Rice (Oryza sativa L.) Aprodu, Iuliana Milea, Ștefania Adelina Anghel, Roxana-Mădălina Enachi, Elena Barbu, Vasilica Crăciunescu, Oana Râpeanu, Gabriela Bahrim, Gabriela Elena Oancea, Anca Stănciuc, Nicoleta Molecules Article The aqueous anthocyanin-rich extract derived from black rice (Oryza sativa L.) was encapsulated by freeze drying using milk proteins and peptides as coating materials. The molecular modelling approach indicated that all major casein fractions and whey proteins were able to bind at least one anthocyanin molecule. The hydrophobic interactions and hydrogen bonding across the interfaces appeared to be mainly responsible for the stabilizations of the complexes formed between the coating material and bioactive compounds. Two dark purple colored powders, differentiated by the ratio of the encapsulation materials used, rich in phytochemicals were obtained, with an encapsulation efficiency of up to 99%. The powders were tested for antioxidant activity, cytocompatibility, and thermal stability. The morphological structure of the powders highlighted the presence of encapsulated anthocyanins. Both powders showed a remarkable antioxidant activity of about 46 mM Trolox/g D.W., and cytocompatibility on the L929 fibroblast culture. At certain concentrations, both powders stimulated cell proliferation. The powders showed a good thermal stability between 75 and 100 °C for 15 min. The powders were tested in a food model system and checked for stability of phytochemicals during storage. The added value of the powders was demonstrated throughout the antioxidant activity, which remained unchanged during storage. MDPI 2019-09-18 /pmc/articles/PMC6766832/ /pubmed/31540422 http://dx.doi.org/10.3390/molecules24183389 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aprodu, Iuliana
Milea, Ștefania Adelina
Anghel, Roxana-Mădălina
Enachi, Elena
Barbu, Vasilica
Crăciunescu, Oana
Râpeanu, Gabriela
Bahrim, Gabriela Elena
Oancea, Anca
Stănciuc, Nicoleta
New Functional Ingredients Based on Microencapsulation of Aqueous Anthocyanin-Rich Extracts Derived from Black Rice (Oryza sativa L.)
title New Functional Ingredients Based on Microencapsulation of Aqueous Anthocyanin-Rich Extracts Derived from Black Rice (Oryza sativa L.)
title_full New Functional Ingredients Based on Microencapsulation of Aqueous Anthocyanin-Rich Extracts Derived from Black Rice (Oryza sativa L.)
title_fullStr New Functional Ingredients Based on Microencapsulation of Aqueous Anthocyanin-Rich Extracts Derived from Black Rice (Oryza sativa L.)
title_full_unstemmed New Functional Ingredients Based on Microencapsulation of Aqueous Anthocyanin-Rich Extracts Derived from Black Rice (Oryza sativa L.)
title_short New Functional Ingredients Based on Microencapsulation of Aqueous Anthocyanin-Rich Extracts Derived from Black Rice (Oryza sativa L.)
title_sort new functional ingredients based on microencapsulation of aqueous anthocyanin-rich extracts derived from black rice (oryza sativa l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766832/
https://www.ncbi.nlm.nih.gov/pubmed/31540422
http://dx.doi.org/10.3390/molecules24183389
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