Titrating Complex Mass Cytometry Panels

We describe here a simple and efficient antibody titration approach for cell‐surface markers and intracellular cell signaling targets for mass cytometry. The iterative approach builds upon a well‐characterized backbone panel of antibodies and analysis using bioinformatic tools such as SPADE. Healthy...

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Detalles Bibliográficos
Autores principales: Gullaksen, Stein‐Erik, Bader, Lucius, Hellesøy, Monica, Sulen, André, Fagerholt, Oda Helen Eck, Engen, Caroline B., Skavland, Jørn, Gjertsen, Bjørn Tore, Gavasso, Sonia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2019
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766997/
https://www.ncbi.nlm.nih.gov/pubmed/30964237
http://dx.doi.org/10.1002/cyto.a.23751

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766997/
https://www.ncbi.nlm.nih.gov/pubmed/30964237
http://dx.doi.org/10.1002/cyto.a.23751

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