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Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

Sugar nucleotide‐dependent (Leloir) glycosyltransferases from plants are important catalysts for the glycosylation of small molecules and natural products. Limitations on their applicability for biocatalytic synthesis arise because of low protein expression (≤10 mg/L culture) in standard microbial h...

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Detalles Bibliográficos
Autores principales: Lemmerer, Martin, Mairhofer, Juergen, Lepak, Alexander, Longus, Karin, Hahn, Rainer, Nidetzky, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767175/
https://www.ncbi.nlm.nih.gov/pubmed/30659592
http://dx.doi.org/10.1002/bit.26934