Cargando…

Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

Sugar nucleotide‐dependent (Leloir) glycosyltransferases from plants are important catalysts for the glycosylation of small molecules and natural products. Limitations on their applicability for biocatalytic synthesis arise because of low protein expression (≤10 mg/L culture) in standard microbial h...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lemmerer, Martin, Mairhofer, Juergen, Lepak, Alexander, Longus, Karin, Hahn, Rainer, Nidetzky, Bernd
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2019
Materias:	ARTICLES
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767175/ https://www.ncbi.nlm.nih.gov/pubmed/30659592 http://dx.doi.org/10.1002/bit.26934

Ejemplares similares

Leloir glycosyltransferases enabled to flow synthesis: Continuous production of the natural C‐glycoside nothofagin
por: Liu, Hui, et al.
Publicado: (2021)

Leloir Glycosyltransferases and Natural Product Glycosylation: Biocatalytic Synthesis of the C-Glucoside Nothofagin, a Major Antioxidant of Redbush Herbal Tea
por: Bungaruang, Linda, et al.
Publicado: (2013)

Selective β-Mono-Glycosylation of a C15-Hydroxylated Metabolite of the Agricultural Herbicide Cinmethylin Using Leloir Glycosyltransferases
por: Jung, Jihye, et al.
Publicado: (2021)

Leloir Glycosyltransferases in Applied Biocatalysis: A Multidisciplinary Approach
por: Mestrom, Luuk, et al.
Publicado: (2019)

Stepwise metabolic adaption from pure metabolization to balanced anaerobic growth on xylose explored for recombinant Saccharomyces cerevisiae
por: Klimacek, Mario, et al.
Publicado: (2014)

In-Depth Characterization of a Re-Engineered Cholera Toxin Manufacturing Process Using Growth-Decoupled Production in Escherichia coli
por: Danielewicz, Natalia, et al.
Publicado: (2022)

From wheat straw to bioethanol: integrative analysis of a separate hydrolysis and co-fermentation process with implemented enzyme production
por: Novy, Vera, et al.
Publicado: (2015)

Expression of the functional recombinant human glycosyltransferase GalNAcT2 in Escherichia coli
por: Lauber, Jennifer, et al.
Publicado: (2015)

Comparison of Scheffersomyces stipitis strains CBS 5773 and CBS 6054 with regard to their xylose metabolism: implications for xylose fermentation
por: Krahulec, Stefan, et al.
Publicado: (2012)

Process intensification through microbial strain evolution: mixed glucose-xylose fermentation in wheat straw hydrolyzates by three generations of recombinant Saccharomyces cerevisiae
por: Novy, Vera, et al.
Publicado: (2014)

The Leloir Cycle in Glioblastoma: Galactose Scavenging and Metabolic Remodeling
por: Sharpe, Martyn A., et al.
Publicado: (2021)

Tunable expression rate control of a growth-decoupled T7 expression system by l-arabinose only
por: Stargardt, Patrick, et al.
Publicado: (2021)

Awakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica
por: Lazar, Zbigniew, et al.
Publicado: (2015)

Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization
por: Krahulec, Stefan, et al.
Publicado: (2010)

d-Xylulose kinase from Saccharomyces cerevisiae: Isolation and characterization of the highly unstable enzyme, recombinantly produced in Escherichia coli
por: Pival, Simone L., et al.
Publicado: (2011)

3D Printed Porous Nanocellulose-Based Scaffolds As Carriers for Immobilization of Glycosyltransferases
por: Lackner, Florian, et al.
Publicado: (2022)

Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production
por: Xue, Feiyan, et al.
Publicado: (2016)

Finished Genome Sequence of Escherichia coli K-12 Strain HMS174 (ATCC 47011)
por: Mairhofer, Juergen, et al.
Publicado: (2014)

Finished Genome Sequence of the Laboratory Strain Escherichia coli K-12 RV308 (ATCC 31608)
por: Krempl, Peter M., et al.
Publicado: (2014)

Higher plant glycosyltransferases
por: Ross, Joe, et al.
Publicado: (2001)

Unexpected NADPH Hydratase Activity in the Nitrile Reductase QueF from Escherichia coli
por: Jung, Jihye, et al.
Publicado: (2020)

Long-term experimental evolution decouples size and production costs in Escherichia coli
por: Marshall, Dustin J., et al.
Publicado: (2022)

Controllable Iterative β-Glucosylation from UDP-Glucose by Bacillus cereus Glycosyltransferase GT1: Application for the Synthesis of Disaccharide-Modified Xenobiotics
por: Jung, Jihye, et al.
Publicado: (2021)

Identification of a Fragment-Based Scaffold that Inhibits the Glycosyltransferase WaaG from Escherichia coli †
por: Muheim, Claudio, et al.
Publicado: (2016)

A Lead-Based Fragment Library Screening of the Glycosyltransferase WaaG from Escherichia coli
por: Riu, Federico, et al.
Publicado: (2022)

A Comparative Analysis of Industrial Escherichia coli K–12 and B Strains in High-Glucose Batch Cultivations on Process-, Transcriptome- and Proteome Level
por: Marisch, Karoline, et al.
Publicado: (2013)

The decoupling between genetic structure and metabolic phenotypes in Escherichia coli leads to continuous phenotypic diversity
por: Sabarly, V, et al.
Publicado: (2011)

Solvent Engineering for Nonpolar Substrate Glycosylation Catalyzed by the UDP-Glucose-Dependent Glycosyltransferase UGT71E5: Intensification of the Synthesis of 15-Hydroxy Cinmethylin β-d-Glucoside
por: Jung, Jihye, et al.
Publicado: (2023)

Whole-cell bioreduction of aromatic α-keto esters using Candida tenuis xylose reductase and Candida boidinii formate dehydrogenase co-expressed in Escherichia coli
por: Kratzer, Regina, et al.
Publicado: (2008)

Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes
por: Welner, Ditte Hededam, et al.
Publicado: (2017)

Optimization of production of recombinant human growth hormone in Escherichia coli
por: Rezaei, Marzieh, et al.
Publicado: (2012)

Inhibition of Escherichia coli glycosyltransferase MurG and Mycobacterium tuberculosis Gal transferase by uridine-linked transition state mimics
por: Trunkfield, Amy E., et al.
Publicado: (2010)

Yihx-encoded haloacid dehalogenase-like phosphatase HAD4 from Escherichia coli is a specific α-d-glucose 1-phosphate hydrolase useful for substrate-selective sugar phosphate transformations
por: Pfeiffer, Martin, et al.
Publicado: (2014)

Structure-function and engineering of plant UDP-glycosyltransferase
por: Wang, Mengya, et al.
Publicado: (2023)

Protocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli
por: Roden, Laura C, et al.
Publicado: (2005)

A comparative approach to recombinantly produce the plant enzyme horseradish peroxidase in Escherichia coli
por: Gundinger, Thomas, et al.
Publicado: (2017)

Expression and Purification of Recombinant Hemoglobin in Escherichia coli
por: Natarajan, Chandrasekhar, et al.
Publicado: (2011)

Hydrogen production by recombinant Escherichia coli strains
por: Maeda, Toshinari, et al.
Publicado: (2012)

Fluorometric In Situ Monitoring of an Escherichia coli Cell Factory with Cytosolic Expression of Human Glycosyltransferase GalNAcT2: Prospects and Limitations
por: Schwab, Karen, et al.
Publicado: (2016)

Biosynthesis of rare 20(R)-protopanaxadiol/protopanaxatriol type ginsenosides through Escherichia coli engineered with uridine diphosphate glycosyltransferase genes
por: Yu, Lu, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_ajlrup5nc6jnbev3ihksl5q0s4