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Characterization of an intertidal zone metagenome oligoribonuclease and the role of the intermolecular disulfide bond for homodimer formation and nuclease activity

The gene encoding MG Orn has been identified from a metagenomic library created from the intertidal zone in Svalbard and encodes a protein of 184 amino acid residues. The mg orn gene has been cloned, recombinantly expressed in Escherichia coli, and purified to homogeneity. Biochemical characterizati...

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Detalles Bibliográficos
Autores principales: Piotrowski, Yvonne, Berg, Kristel, Klebl, David Paul, Leiros, Ingar, Larsen, Atle Noralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768110/
https://www.ncbi.nlm.nih.gov/pubmed/31420950
http://dx.doi.org/10.1002/2211-5463.12720