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Effects of Lyse-It on endonuclease fragmentation, function and activity

Nucleases are enzymes that can degrade genomic DNA and RNA that decrease the accuracy of quantitative measures of those nucleic acids. Here, we study conventional heating, standard microwave irradiation, and Lyse-It, a microwave-based lysing technology, for the potential to fragment and inactivate D...

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Detalles Bibliográficos
Autores principales: Santaus, Tonya M., Zhang, Fan, Li, Shan, Stine, O. Colin, Geddes, Chris D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768537/
https://www.ncbi.nlm.nih.gov/pubmed/31568482
http://dx.doi.org/10.1371/journal.pone.0223008