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Assessing Ploidy Level Analysis and Single Pollen Genotyping of Diploid and Euploid Citrus Genotypes by Fluorescence-Activated Cell Sorting and Whole-Genome Amplification

Flow cytometry is widely used to determine genome size and ploidy level in plants. This technique, when coupled with fluorescence-activated cell sorting (FACS), whole genome amplification and genotyping (WGA), opens up new opportunities for genetic studies of individualized nuclei. This strategy was...

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Detalles Bibliográficos
Autores principales: Garavello, Miguel, Cuenca, José, Dreissig, Steven, Fuchs, Jörg, Houben, Andreas, Aleza, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769063/
https://www.ncbi.nlm.nih.gov/pubmed/31611896
http://dx.doi.org/10.3389/fpls.2019.01174