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Off‐Pathway‐Sensitive Protein‐Splicing Screening Based on a Toxin/Antitoxin System

Protein‐splicing domains are frequently used engineering tools that find application in the in vivo and in vitro ligation of protein domains. Directed evolution is among the most promising technologies used to advance this technology. However, the available screening systems for protein‐splicing act...

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Detalles Bibliográficos
Autores principales: Beyer, Hannes M., Iwaï, Hideo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771659/
https://www.ncbi.nlm.nih.gov/pubmed/30963690
http://dx.doi.org/10.1002/cbic.201900139