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Off‐Pathway‐Sensitive Protein‐Splicing Screening Based on a Toxin/Antitoxin System
Protein‐splicing domains are frequently used engineering tools that find application in the in vivo and in vitro ligation of protein domains. Directed evolution is among the most promising technologies used to advance this technology. However, the available screening systems for protein‐splicing act...
Autores principales: | Beyer, Hannes M., Iwaï, Hideo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771659/ https://www.ncbi.nlm.nih.gov/pubmed/30963690 http://dx.doi.org/10.1002/cbic.201900139 |
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