Pichia pastoris protease‐deficient and auxotrophic strains generated by a novel, user‐friendly vector toolbox for gene deletion

Targeted gene knockouts play an important role in the study of gene function. For the generation of knockouts in the industrially important yeast Pichia pastoris, several protocols have been published to date. Nevertheless, creating a targeted knockout in P. pastoris still is a time‐consuming proces...

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Detalles Bibliográficos
Autores principales: Ahmad, Mudassar, Winkler, Christine M., Kolmbauer, Markus, Pichler, Harald, Schwab, Helmut, Emmerstorfer‐Augustin, Anita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771850/
https://www.ncbi.nlm.nih.gov/pubmed/31148217
http://dx.doi.org/10.1002/yea.3426

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771850/
https://www.ncbi.nlm.nih.gov/pubmed/31148217
http://dx.doi.org/10.1002/yea.3426

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