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Type I toxin-dependent generation of superoxide affects the persister life cycle of Escherichia coli
Induction of growth stasis by bacterial toxins from chromosomal toxin-antitoxin systems is suspected to favor formation of multidrug-tolerant cells, named persisters. Recurrent infections are often attributed to resuscitation and regrowth of persisters upon termination of antibiotic therapy. Several...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776643/ https://www.ncbi.nlm.nih.gov/pubmed/31582786 http://dx.doi.org/10.1038/s41598-019-50668-1 |
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author | Edelmann, Daniel Berghoff, Bork A. |
author_facet | Edelmann, Daniel Berghoff, Bork A. |
author_sort | Edelmann, Daniel |
collection | PubMed |
description | Induction of growth stasis by bacterial toxins from chromosomal toxin-antitoxin systems is suspected to favor formation of multidrug-tolerant cells, named persisters. Recurrent infections are often attributed to resuscitation and regrowth of persisters upon termination of antibiotic therapy. Several lines of evidence point to oxidative stress as a crucial factor during the persister life cycle. Here, we demonstrate that the membrane-depolarizing type I toxins TisB, DinQ, and HokB have the potential to provoke reactive oxygen species formation in Escherichia coli. More detailed work with TisB revealed that mainly superoxide is formed, leading to activation of the SoxRS regulon. Deletion of the genes encoding the cytoplasmic superoxide dismutases SodA and SodB caused both a decline in TisB-dependent persisters and a delay in persister recovery upon termination of antibiotic treatment. We hypothesize that expression of depolarizing toxins during the persister formation process inflicts an oxidative challenge. The ability to counteract oxidative stress might determine whether cells will survive and how much time they need to recover from dormancy. |
format | Online Article Text |
id | pubmed-6776643 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67766432019-10-09 Type I toxin-dependent generation of superoxide affects the persister life cycle of Escherichia coli Edelmann, Daniel Berghoff, Bork A. Sci Rep Article Induction of growth stasis by bacterial toxins from chromosomal toxin-antitoxin systems is suspected to favor formation of multidrug-tolerant cells, named persisters. Recurrent infections are often attributed to resuscitation and regrowth of persisters upon termination of antibiotic therapy. Several lines of evidence point to oxidative stress as a crucial factor during the persister life cycle. Here, we demonstrate that the membrane-depolarizing type I toxins TisB, DinQ, and HokB have the potential to provoke reactive oxygen species formation in Escherichia coli. More detailed work with TisB revealed that mainly superoxide is formed, leading to activation of the SoxRS regulon. Deletion of the genes encoding the cytoplasmic superoxide dismutases SodA and SodB caused both a decline in TisB-dependent persisters and a delay in persister recovery upon termination of antibiotic treatment. We hypothesize that expression of depolarizing toxins during the persister formation process inflicts an oxidative challenge. The ability to counteract oxidative stress might determine whether cells will survive and how much time they need to recover from dormancy. Nature Publishing Group UK 2019-10-03 /pmc/articles/PMC6776643/ /pubmed/31582786 http://dx.doi.org/10.1038/s41598-019-50668-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Edelmann, Daniel Berghoff, Bork A. Type I toxin-dependent generation of superoxide affects the persister life cycle of Escherichia coli |
title | Type I toxin-dependent generation of superoxide affects the persister life cycle of Escherichia coli |
title_full | Type I toxin-dependent generation of superoxide affects the persister life cycle of Escherichia coli |
title_fullStr | Type I toxin-dependent generation of superoxide affects the persister life cycle of Escherichia coli |
title_full_unstemmed | Type I toxin-dependent generation of superoxide affects the persister life cycle of Escherichia coli |
title_short | Type I toxin-dependent generation of superoxide affects the persister life cycle of Escherichia coli |
title_sort | type i toxin-dependent generation of superoxide affects the persister life cycle of escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776643/ https://www.ncbi.nlm.nih.gov/pubmed/31582786 http://dx.doi.org/10.1038/s41598-019-50668-1 |
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