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De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
High-throughput CRISPR-Cas9 knockout screens using a tiling-sgRNA design permit in situ evaluation of protein domain function. Here, to facilitate de novo identification of essential protein domains from such screens, we propose ProTiler, a computational method for the robust mapping of CRISPR knock...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778102/ https://www.ncbi.nlm.nih.gov/pubmed/31586052 http://dx.doi.org/10.1038/s41467-019-12489-8 |