High-fidelity endonuclease variant HypaCas9 facilitates accurate allele-specific gene modification in mouse zygotes

CRISPR/Cas9 has been widely used for the efficient generation of genetically modified animals; however, this system could have unexpected off-target effects. In the present study, we confirmed the validity of a high-fidelity Cas9 variant, HypaCas9, for accurate genome editing in mouse zygotes. HypaC...

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Detalles Bibliográficos
Autores principales: Ikeda, Arisa, Fujii, Wataru, Sugiura, Koji, Naito, Kunihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787007/
https://www.ncbi.nlm.nih.gov/pubmed/31633062
http://dx.doi.org/10.1038/s42003-019-0627-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787007/
https://www.ncbi.nlm.nih.gov/pubmed/31633062
http://dx.doi.org/10.1038/s42003-019-0627-8

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