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In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II

Chlorine dioxide (ClO(2)) has been widely used as an effective disinfectant to control fungal contamination during postharvest crop storage. In this study, Fusarium oxysporum f. sp. batatas SP-f6 from the black rot symptom of sweetpotato was isolated and identified using phylogenetic analysis of elo...

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Autores principales: Lee, Ye Ji, Jeong, Jin-Ju, Jin, Hyunjung, Kim, Wook, Jeun, Young Chull, Yu, Gyeong-Dan, Kim, Ki Deok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788411/
https://www.ncbi.nlm.nih.gov/pubmed/31632219
http://dx.doi.org/10.5423/PPJ.OA.04.2019.0078
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author Lee, Ye Ji
Jeong, Jin-Ju
Jin, Hyunjung
Kim, Wook
Jeun, Young Chull
Yu, Gyeong-Dan
Kim, Ki Deok
author_facet Lee, Ye Ji
Jeong, Jin-Ju
Jin, Hyunjung
Kim, Wook
Jeun, Young Chull
Yu, Gyeong-Dan
Kim, Ki Deok
author_sort Lee, Ye Ji
collection PubMed
description Chlorine dioxide (ClO(2)) has been widely used as an effective disinfectant to control fungal contamination during postharvest crop storage. In this study, Fusarium oxysporum f. sp. batatas SP-f6 from the black rot symptom of sweetpotato was isolated and identified using phylogenetic analysis of elongation factor 1-α gene; we further examined the in vitro and in vivo inhibitory activities of ClO(2) gas against the fungus. In the in vitro medium tests, fungal population was significantly inhibited upon increasing the concentration and exposure time. In in vivo tests, spore suspensions were drop-inoculated onto sweetpotato slices, followed by treatment using various ClO(2) concentrations and treatment times to assess fungus-induced disease development in the slices. Lesion diameters decreased at the tested ClO(2) concentrations over time. When sweetpotato roots were dip-inoculated in spore suspensions prior to treatment with 20 and 40 ppm of ClO(2) for 0–60 min, fungal populations significantly decreased at the tested concentrations for 30–60 min. Taken together, these results showed that ClO(2) gas can effectively inhibit fungal growth and disease development caused by F. oxysporum f. sp. batatas on sweetpotato. Therefore, ClO(2) gas may be used as a sanitizer to control this fungus during postharvest storage of sweetpotato.
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spelling pubmed-67884112019-10-18 In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II Lee, Ye Ji Jeong, Jin-Ju Jin, Hyunjung Kim, Wook Jeun, Young Chull Yu, Gyeong-Dan Kim, Ki Deok Plant Pathol J Research Article Chlorine dioxide (ClO(2)) has been widely used as an effective disinfectant to control fungal contamination during postharvest crop storage. In this study, Fusarium oxysporum f. sp. batatas SP-f6 from the black rot symptom of sweetpotato was isolated and identified using phylogenetic analysis of elongation factor 1-α gene; we further examined the in vitro and in vivo inhibitory activities of ClO(2) gas against the fungus. In the in vitro medium tests, fungal population was significantly inhibited upon increasing the concentration and exposure time. In in vivo tests, spore suspensions were drop-inoculated onto sweetpotato slices, followed by treatment using various ClO(2) concentrations and treatment times to assess fungus-induced disease development in the slices. Lesion diameters decreased at the tested ClO(2) concentrations over time. When sweetpotato roots were dip-inoculated in spore suspensions prior to treatment with 20 and 40 ppm of ClO(2) for 0–60 min, fungal populations significantly decreased at the tested concentrations for 30–60 min. Taken together, these results showed that ClO(2) gas can effectively inhibit fungal growth and disease development caused by F. oxysporum f. sp. batatas on sweetpotato. Therefore, ClO(2) gas may be used as a sanitizer to control this fungus during postharvest storage of sweetpotato. Korean Society of Plant Pathology 2019-10 2019-10-01 /pmc/articles/PMC6788411/ /pubmed/31632219 http://dx.doi.org/10.5423/PPJ.OA.04.2019.0078 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lee, Ye Ji
Jeong, Jin-Ju
Jin, Hyunjung
Kim, Wook
Jeun, Young Chull
Yu, Gyeong-Dan
Kim, Ki Deok
In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II
title In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II
title_full In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II
title_fullStr In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II
title_full_unstemmed In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II
title_short In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II
title_sort in vitro and in vivo inhibitory effects of gaseous chlorine dioxide against fusarium oxysporum f. sp. batatas isolated from stored sweetpotato: study ii
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788411/
https://www.ncbi.nlm.nih.gov/pubmed/31632219
http://dx.doi.org/10.5423/PPJ.OA.04.2019.0078
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