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Mitochondrial reactive oxygen species generation in blood cells is associated with disease severity and exercise intolerance in heart failure patients

Systemic oxidative stress plays a key role in the development of chronic heart failure (CHF). We tested the hypothesis that mitochondrial reactive oxygen species (ROS) generation in circulating peripheral blood mononuclear cells (PBMCs) contributes to CHF progression. A total of 31 patients who had...

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Autores principales: Shirakawa, Ryosuke, Yokota, Takashi, Nakajima, Takayuki, Takada, Shingo, Yamane, Miwako, Furihata, Takaaki, Maekawa, Satoshi, Nambu, Hideo, Katayama, Takashi, Fukushima, Arata, Saito, Akimichi, Ishimori, Naoki, Dela, Flemming, Kinugawa, Shintaro, Anzai, Toshihisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789126/
https://www.ncbi.nlm.nih.gov/pubmed/31605012
http://dx.doi.org/10.1038/s41598-019-51298-3
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author Shirakawa, Ryosuke
Yokota, Takashi
Nakajima, Takayuki
Takada, Shingo
Yamane, Miwako
Furihata, Takaaki
Maekawa, Satoshi
Nambu, Hideo
Katayama, Takashi
Fukushima, Arata
Saito, Akimichi
Ishimori, Naoki
Dela, Flemming
Kinugawa, Shintaro
Anzai, Toshihisa
author_facet Shirakawa, Ryosuke
Yokota, Takashi
Nakajima, Takayuki
Takada, Shingo
Yamane, Miwako
Furihata, Takaaki
Maekawa, Satoshi
Nambu, Hideo
Katayama, Takashi
Fukushima, Arata
Saito, Akimichi
Ishimori, Naoki
Dela, Flemming
Kinugawa, Shintaro
Anzai, Toshihisa
author_sort Shirakawa, Ryosuke
collection PubMed
description Systemic oxidative stress plays a key role in the development of chronic heart failure (CHF). We tested the hypothesis that mitochondrial reactive oxygen species (ROS) generation in circulating peripheral blood mononuclear cells (PBMCs) contributes to CHF progression. A total of 31 patients who had a history of hospital admission due to worsening HF were enrolled and grouped as having either mild CHF defined as New York Heart Association (NYHA) functional class I-II or moderate-to-severe CHF defined as NYHA functional class III. ROS levels in PBMC mitochondria were significantly increased in CHF patients with NYHA functional class III compared to those with NYHA functional class I-II, accompanied by impaired mitochondrial respiratory capacity in PBMCs. ROS generation in PBMC mitochondria was positively correlated with urinary 8-hydroxydeoxyguanosine, a systemic oxidative stress marker, in CHF patients. Importantly, mitochondrial ROS generation in PBMCs was directly correlated with plasma levels of B-type natriuretic peptide, a biomarker for severity of HF, and inversely correlated with peak oxygen uptake, a parameter of exercise capacity, in CHF patients. The study showed that ROS generation in PBMC mitochondria was higher in patients with advanced CHF, and it was associated with disease severity and exercise intolerance in CHF patients.
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spelling pubmed-67891262019-10-17 Mitochondrial reactive oxygen species generation in blood cells is associated with disease severity and exercise intolerance in heart failure patients Shirakawa, Ryosuke Yokota, Takashi Nakajima, Takayuki Takada, Shingo Yamane, Miwako Furihata, Takaaki Maekawa, Satoshi Nambu, Hideo Katayama, Takashi Fukushima, Arata Saito, Akimichi Ishimori, Naoki Dela, Flemming Kinugawa, Shintaro Anzai, Toshihisa Sci Rep Article Systemic oxidative stress plays a key role in the development of chronic heart failure (CHF). We tested the hypothesis that mitochondrial reactive oxygen species (ROS) generation in circulating peripheral blood mononuclear cells (PBMCs) contributes to CHF progression. A total of 31 patients who had a history of hospital admission due to worsening HF were enrolled and grouped as having either mild CHF defined as New York Heart Association (NYHA) functional class I-II or moderate-to-severe CHF defined as NYHA functional class III. ROS levels in PBMC mitochondria were significantly increased in CHF patients with NYHA functional class III compared to those with NYHA functional class I-II, accompanied by impaired mitochondrial respiratory capacity in PBMCs. ROS generation in PBMC mitochondria was positively correlated with urinary 8-hydroxydeoxyguanosine, a systemic oxidative stress marker, in CHF patients. Importantly, mitochondrial ROS generation in PBMCs was directly correlated with plasma levels of B-type natriuretic peptide, a biomarker for severity of HF, and inversely correlated with peak oxygen uptake, a parameter of exercise capacity, in CHF patients. The study showed that ROS generation in PBMC mitochondria was higher in patients with advanced CHF, and it was associated with disease severity and exercise intolerance in CHF patients. Nature Publishing Group UK 2019-10-11 /pmc/articles/PMC6789126/ /pubmed/31605012 http://dx.doi.org/10.1038/s41598-019-51298-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shirakawa, Ryosuke
Yokota, Takashi
Nakajima, Takayuki
Takada, Shingo
Yamane, Miwako
Furihata, Takaaki
Maekawa, Satoshi
Nambu, Hideo
Katayama, Takashi
Fukushima, Arata
Saito, Akimichi
Ishimori, Naoki
Dela, Flemming
Kinugawa, Shintaro
Anzai, Toshihisa
Mitochondrial reactive oxygen species generation in blood cells is associated with disease severity and exercise intolerance in heart failure patients
title Mitochondrial reactive oxygen species generation in blood cells is associated with disease severity and exercise intolerance in heart failure patients
title_full Mitochondrial reactive oxygen species generation in blood cells is associated with disease severity and exercise intolerance in heart failure patients
title_fullStr Mitochondrial reactive oxygen species generation in blood cells is associated with disease severity and exercise intolerance in heart failure patients
title_full_unstemmed Mitochondrial reactive oxygen species generation in blood cells is associated with disease severity and exercise intolerance in heart failure patients
title_short Mitochondrial reactive oxygen species generation in blood cells is associated with disease severity and exercise intolerance in heart failure patients
title_sort mitochondrial reactive oxygen species generation in blood cells is associated with disease severity and exercise intolerance in heart failure patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789126/
https://www.ncbi.nlm.nih.gov/pubmed/31605012
http://dx.doi.org/10.1038/s41598-019-51298-3
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