Simple and large-scale chromosomal engineering of mouse zygotes via in vitro and in vivo electroporation

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has facilitated dramatic progress in the field of genome engineering. Whilst microinjection of the Cas9 protein and a single guide RNA (sgRNA) into mouse zygotes is a widespread method for producing genetically engine...

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Detalles Bibliográficos
Autores principales: Iwata, Satoru, Nakadai, Hitomi, Fukushi, Daisuke, Jose, Mami, Nagahara, Miki, Iwamoto, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789149/
https://www.ncbi.nlm.nih.gov/pubmed/31604975
http://dx.doi.org/10.1038/s41598-019-50900-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789149/
https://www.ncbi.nlm.nih.gov/pubmed/31604975
http://dx.doi.org/10.1038/s41598-019-50900-y

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