Imaging endogenous synaptic proteins in primary neurons at single-cell resolution using CRISPR/Cas9

Fluorescence imaging at single-cell resolution is a crucial approach to analyzing the spatiotemporal regulation of proteins within individual cells of complex neural networks. Here we present a nonviral strategy that enables the tagging of endogenous loci by CRISPR/Cas9-mediated genome editing combi...

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Detalles Bibliográficos
Autores principales: Matsuda, Takahiko, Oinuma, Izumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789158/
https://www.ncbi.nlm.nih.gov/pubmed/31509485
http://dx.doi.org/10.1091/mbc.E19-04-0223

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789158/
https://www.ncbi.nlm.nih.gov/pubmed/31509485
http://dx.doi.org/10.1091/mbc.E19-04-0223

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