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Imaging endogenous synaptic proteins in primary neurons at single-cell resolution using CRISPR/Cas9
Fluorescence imaging at single-cell resolution is a crucial approach to analyzing the spatiotemporal regulation of proteins within individual cells of complex neural networks. Here we present a nonviral strategy that enables the tagging of endogenous loci by CRISPR/Cas9-mediated genome editing combi...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The American Society for Cell Biology
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789158/ https://www.ncbi.nlm.nih.gov/pubmed/31509485 http://dx.doi.org/10.1091/mbc.E19-04-0223 |