A Crude Extract Preparation and Optimization from a Genomically Engineered Escherichia coli for the Cell-Free Protein Synthesis System: Practical Laboratory Guideline

With the advancement of synthetic biology, the cell-free protein synthesis (CFPS) system has been receiving the spotlight as a versatile toolkit for engineering natural and unnatural biological systems. The CFPS system reassembles the materials necessary for transcription and translation and recreat...

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Detalles Bibliográficos
Autores principales: Kim, Jeehye, Copeland, Caroline E., Padumane, Sahana R., Kwon, Yong-Chan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789667/
https://www.ncbi.nlm.nih.gov/pubmed/31405077
http://dx.doi.org/10.3390/mps2030068

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789667/
https://www.ncbi.nlm.nih.gov/pubmed/31405077
http://dx.doi.org/10.3390/mps2030068

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