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Versatile transcription control based on reversible dCas9 binding

The ability to control transcription in a time-dependent manner in vitro promises numerous applications in molecular biology and nanotechnology. Here we demonstrate an approach that enables precise, independent control over the production of multiple RNA transcripts in vitro using single guide RNA (...

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Detalles Bibliográficos
Autores principales: Widom, Julia R., Rai, Victoria, Rohlman, Christopher E., Walter, Nils G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6795147/
https://www.ncbi.nlm.nih.gov/pubmed/31320398
http://dx.doi.org/10.1261/rna.071613.119