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Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop

The present study mainly consists of a re-evaluation of the rate at which C(12)E(8), a typical non-ionic detergent used for membrane studies, is able to dissociate from biological membranes, with sarcoplasmic reticulum membrane vesicles being used as an example. Utilizing a brominated derivative of...

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Detalles Bibliográficos
Autores principales: Champeil, Philippe, de Foresta, Béatrice, Picard, Martin, Gauron, Carole, Georgin, Dominique, le Maire, Marc, Møller, Jesper V., Lenoir, Guillaume, Montigny, Cédric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6795424/
https://www.ncbi.nlm.nih.gov/pubmed/31618200
http://dx.doi.org/10.1371/journal.pone.0222932