Generation of deletions and precise point mutations in Dictyostelium discoideum using the CRISPR nickase

The CRISPR/Cas9 system enables targeted genome modifications across a range of eukaryotes. Although we have reported that transient introduction of all-in-one vectors that express both Cas9 and sgRNAs can efficiently induce multiple gene knockouts in Dictyostelium discoideum, concerns remain about o...

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Detalles Bibliográficos
Autores principales: Iriki, Hoshie, Kawata, Takefumi, Muramoto, Tetsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797129/
https://www.ncbi.nlm.nih.gov/pubmed/31622451
http://dx.doi.org/10.1371/journal.pone.0224128

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797129/
https://www.ncbi.nlm.nih.gov/pubmed/31622451
http://dx.doi.org/10.1371/journal.pone.0224128

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