Cargando…
Generation of deletions and precise point mutations in Dictyostelium discoideum using the CRISPR nickase
The CRISPR/Cas9 system enables targeted genome modifications across a range of eukaryotes. Although we have reported that transient introduction of all-in-one vectors that express both Cas9 and sgRNAs can efficiently induce multiple gene knockouts in Dictyostelium discoideum, concerns remain about o...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2019
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797129/ https://www.ncbi.nlm.nih.gov/pubmed/31622451 http://dx.doi.org/10.1371/journal.pone.0224128 |