Analysis of Single Nucleotide Variants in CRISPR-Cas9 Edited Zebrafish Exomes Shows No Evidence of Off-Target Inflation

Therapeutic applications of CRISPR-Cas9 gene editing have spurred innovation in Cas9 enzyme engineering and single guide RNA (sgRNA) design algorithms to minimize potential off-target events. While recent work in rodents outlines favorable conditions for specific editing and uses a trio design (moth...

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Detalles Bibliográficos
Autores principales: Mooney, Marie R., Davis, Erica E., Katsanis, Nicholas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797590/
https://www.ncbi.nlm.nih.gov/pubmed/31681410
http://dx.doi.org/10.3389/fgene.2019.00949

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797590/
https://www.ncbi.nlm.nih.gov/pubmed/31681410
http://dx.doi.org/10.3389/fgene.2019.00949

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