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PTEN modulates neurites outgrowth and neuron apoptosis involving the PI3K/Akt/mTOR signaling pathway

The present study aimed to explore the role of the PTEN/Akt/mTOR signaling pathway in the neurite outgrowth and apoptosis of cortical neurons. Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of the neurites were calculated. I...

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Autores principales: Liu, Shen, Jia, Jun, Zhou, Hengxing, Zhang, Chi, Liu, Lu, Liu, Jun, Lu, Lu, Li, Xueying, Kang, Yi, Lou, Yongfu, Cai, Zhiwei, Ren, Yiming, Kong, Xiaohong, Feng, Shiqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797942/
https://www.ncbi.nlm.nih.gov/pubmed/31702028
http://dx.doi.org/10.3892/mmr.2019.10670
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author Liu, Shen
Jia, Jun
Zhou, Hengxing
Zhang, Chi
Liu, Lu
Liu, Jun
Lu, Lu
Li, Xueying
Kang, Yi
Lou, Yongfu
Cai, Zhiwei
Ren, Yiming
Kong, Xiaohong
Feng, Shiqing
author_facet Liu, Shen
Jia, Jun
Zhou, Hengxing
Zhang, Chi
Liu, Lu
Liu, Jun
Lu, Lu
Li, Xueying
Kang, Yi
Lou, Yongfu
Cai, Zhiwei
Ren, Yiming
Kong, Xiaohong
Feng, Shiqing
author_sort Liu, Shen
collection PubMed
description The present study aimed to explore the role of the PTEN/Akt/mTOR signaling pathway in the neurite outgrowth and apoptosis of cortical neurons. Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of the neurites were calculated. Immunohistochemical staining and TUNEL data were analyzed. Neurites treated with PTEN inhibitor exhibited significant enhancements in elongation, initiation and crossing abilities when they encountered chondroitin sulfate proteoglycans in vitro. These effects disappeared when the PTEN/Akt/mTOR signaling pathway was blocked. Neurons exhibited significant enhancements in survival ability following PTEN inhibition. The present study demonstrated that PTEN inhibition can promote axonal elongation and initiation in cerebral cortical neurons, as well as the ability to cross the chondroitin sulfate proteoglycan border. In addition, PTEN inhibition is useful for protecting the neuron from apoptosis. The PTEN/Akt/mTOR signaling pathway is an important signaling pathway.
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spelling pubmed-67979422019-10-22 PTEN modulates neurites outgrowth and neuron apoptosis involving the PI3K/Akt/mTOR signaling pathway Liu, Shen Jia, Jun Zhou, Hengxing Zhang, Chi Liu, Lu Liu, Jun Lu, Lu Li, Xueying Kang, Yi Lou, Yongfu Cai, Zhiwei Ren, Yiming Kong, Xiaohong Feng, Shiqing Mol Med Rep Articles The present study aimed to explore the role of the PTEN/Akt/mTOR signaling pathway in the neurite outgrowth and apoptosis of cortical neurons. Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of the neurites were calculated. Immunohistochemical staining and TUNEL data were analyzed. Neurites treated with PTEN inhibitor exhibited significant enhancements in elongation, initiation and crossing abilities when they encountered chondroitin sulfate proteoglycans in vitro. These effects disappeared when the PTEN/Akt/mTOR signaling pathway was blocked. Neurons exhibited significant enhancements in survival ability following PTEN inhibition. The present study demonstrated that PTEN inhibition can promote axonal elongation and initiation in cerebral cortical neurons, as well as the ability to cross the chondroitin sulfate proteoglycan border. In addition, PTEN inhibition is useful for protecting the neuron from apoptosis. The PTEN/Akt/mTOR signaling pathway is an important signaling pathway. D.A. Spandidos 2019-11 2019-09-10 /pmc/articles/PMC6797942/ /pubmed/31702028 http://dx.doi.org/10.3892/mmr.2019.10670 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Shen
Jia, Jun
Zhou, Hengxing
Zhang, Chi
Liu, Lu
Liu, Jun
Lu, Lu
Li, Xueying
Kang, Yi
Lou, Yongfu
Cai, Zhiwei
Ren, Yiming
Kong, Xiaohong
Feng, Shiqing
PTEN modulates neurites outgrowth and neuron apoptosis involving the PI3K/Akt/mTOR signaling pathway
title PTEN modulates neurites outgrowth and neuron apoptosis involving the PI3K/Akt/mTOR signaling pathway
title_full PTEN modulates neurites outgrowth and neuron apoptosis involving the PI3K/Akt/mTOR signaling pathway
title_fullStr PTEN modulates neurites outgrowth and neuron apoptosis involving the PI3K/Akt/mTOR signaling pathway
title_full_unstemmed PTEN modulates neurites outgrowth and neuron apoptosis involving the PI3K/Akt/mTOR signaling pathway
title_short PTEN modulates neurites outgrowth and neuron apoptosis involving the PI3K/Akt/mTOR signaling pathway
title_sort pten modulates neurites outgrowth and neuron apoptosis involving the pi3k/akt/mtor signaling pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797942/
https://www.ncbi.nlm.nih.gov/pubmed/31702028
http://dx.doi.org/10.3892/mmr.2019.10670
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