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Comprehensive in vivo identification of the c-Myc mRNA protein interactome using HyPR-MS

Proteins bind mRNA through their entire life cycle from transcription to degradation. We analyzed c-Myc mRNA protein interactors in vivo using the HyPR-MS method to capture the crosslinked mRNA by hybridization and then analyzed the bound proteins using mass spectrometry proteomics. Using HyPR-MS, 2...

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Detalles Bibliográficos
Autores principales: Spiniello, Michele, Steinbrink, Maisie I., Cesnik, Anthony J., Miller, Rachel M., Scalf, Mark, Shortreed, Michael R., Smith, Lloyd M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6800478/
https://www.ncbi.nlm.nih.gov/pubmed/31296583
http://dx.doi.org/10.1261/rna.072157.119