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SplintQuant: a method for accurately quantifying circular RNA transcript abundance without reverse transcription bias
Reverse transcription of RNA is fallible, introducing biases and confounding the quantification of transcript abundance. We demonstrate that circular RNAs (circRNAs) are more subjective to overestimation of transcript abundance than cognate linear RNAs due to their covalently closed, circular form,...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor Laboratory Press
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6800515/ https://www.ncbi.nlm.nih.gov/pubmed/31151991 http://dx.doi.org/10.1261/rna.070953.119 |