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New tools for high‐throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastoris
Heterologous protein expression in yeast, mostly in Saccharomyces cerevisiae and Pichia pastoris, is a well‐established and widely used technique. It typically requires the construction of an expression vector in Escherichia coli containing the foreign gene and its subsequent transformation into yea...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801181/ https://www.ncbi.nlm.nih.gov/pubmed/30289201 http://dx.doi.org/10.1111/1751-7915.13322 |