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New tools for high‐throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastoris

Heterologous protein expression in yeast, mostly in Saccharomyces cerevisiae and Pichia pastoris, is a well‐established and widely used technique. It typically requires the construction of an expression vector in Escherichia coli containing the foreign gene and its subsequent transformation into yea...

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Detalles Bibliográficos
Autores principales: González, Mario, Brito, Nélida, Hernández‐Bolaños, Eduardo, González, Celedonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801181/
https://www.ncbi.nlm.nih.gov/pubmed/30289201
http://dx.doi.org/10.1111/1751-7915.13322