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A Novel HDL-Mimetic Peptide HM-10/10 Protects RPE and Photoreceptors in Murine Models of Retinal Degeneration
Age-related macular degeneration (AMD) is a leading cause of blindness in the developed world. The retinal pigment epithelium (RPE) is a critical site of pathology in AMD. Oxidative stress plays a key role in the development of AMD. We generated a chimeric high-density lipoprotein (HDL), mimetic pep...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801888/ https://www.ncbi.nlm.nih.gov/pubmed/31569695 http://dx.doi.org/10.3390/ijms20194807 |
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author | Su, Feng Spee, Christine Araujo, Eduardo Barron, Eric Wang, Mo Ghione, Caleb Hinton, David R. Nusinowitz, Steven Kannan, Ram Reddy, Srinivasa T. Farias-Eisner, Robin |
author_facet | Su, Feng Spee, Christine Araujo, Eduardo Barron, Eric Wang, Mo Ghione, Caleb Hinton, David R. Nusinowitz, Steven Kannan, Ram Reddy, Srinivasa T. Farias-Eisner, Robin |
author_sort | Su, Feng |
collection | PubMed |
description | Age-related macular degeneration (AMD) is a leading cause of blindness in the developed world. The retinal pigment epithelium (RPE) is a critical site of pathology in AMD. Oxidative stress plays a key role in the development of AMD. We generated a chimeric high-density lipoprotein (HDL), mimetic peptide named HM-10/10, with anti-oxidant properties and investigated its potential for the treatment of retinal disease using cell culture and animal models of RPE and photoreceptor (PR) degeneration. Treatment with HM-10/10 peptide prevented human fetal RPE cell death caused by tert-Butyl hydroperoxide (tBH)-induced oxidative stress and sodium iodate (NaIO(3)), which causes RPE atrophy and is a model of geographic atrophy in mice. We also show that HM-10/10 peptide ameliorated photoreceptor cell death and significantly improved retinal function in a mouse model of N-methyl-N-nitrosourea (MNU)-induced PR degeneration. Our results demonstrate that HM-10/10 protects RPE and retina from oxidant injury and can serve as a potential therapeutic agent for the treatment of retinal degeneration. |
format | Online Article Text |
id | pubmed-6801888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68018882019-10-31 A Novel HDL-Mimetic Peptide HM-10/10 Protects RPE and Photoreceptors in Murine Models of Retinal Degeneration Su, Feng Spee, Christine Araujo, Eduardo Barron, Eric Wang, Mo Ghione, Caleb Hinton, David R. Nusinowitz, Steven Kannan, Ram Reddy, Srinivasa T. Farias-Eisner, Robin Int J Mol Sci Article Age-related macular degeneration (AMD) is a leading cause of blindness in the developed world. The retinal pigment epithelium (RPE) is a critical site of pathology in AMD. Oxidative stress plays a key role in the development of AMD. We generated a chimeric high-density lipoprotein (HDL), mimetic peptide named HM-10/10, with anti-oxidant properties and investigated its potential for the treatment of retinal disease using cell culture and animal models of RPE and photoreceptor (PR) degeneration. Treatment with HM-10/10 peptide prevented human fetal RPE cell death caused by tert-Butyl hydroperoxide (tBH)-induced oxidative stress and sodium iodate (NaIO(3)), which causes RPE atrophy and is a model of geographic atrophy in mice. We also show that HM-10/10 peptide ameliorated photoreceptor cell death and significantly improved retinal function in a mouse model of N-methyl-N-nitrosourea (MNU)-induced PR degeneration. Our results demonstrate that HM-10/10 protects RPE and retina from oxidant injury and can serve as a potential therapeutic agent for the treatment of retinal degeneration. MDPI 2019-09-27 /pmc/articles/PMC6801888/ /pubmed/31569695 http://dx.doi.org/10.3390/ijms20194807 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Su, Feng Spee, Christine Araujo, Eduardo Barron, Eric Wang, Mo Ghione, Caleb Hinton, David R. Nusinowitz, Steven Kannan, Ram Reddy, Srinivasa T. Farias-Eisner, Robin A Novel HDL-Mimetic Peptide HM-10/10 Protects RPE and Photoreceptors in Murine Models of Retinal Degeneration |
title | A Novel HDL-Mimetic Peptide HM-10/10 Protects RPE and Photoreceptors in Murine Models of Retinal Degeneration |
title_full | A Novel HDL-Mimetic Peptide HM-10/10 Protects RPE and Photoreceptors in Murine Models of Retinal Degeneration |
title_fullStr | A Novel HDL-Mimetic Peptide HM-10/10 Protects RPE and Photoreceptors in Murine Models of Retinal Degeneration |
title_full_unstemmed | A Novel HDL-Mimetic Peptide HM-10/10 Protects RPE and Photoreceptors in Murine Models of Retinal Degeneration |
title_short | A Novel HDL-Mimetic Peptide HM-10/10 Protects RPE and Photoreceptors in Murine Models of Retinal Degeneration |
title_sort | novel hdl-mimetic peptide hm-10/10 protects rpe and photoreceptors in murine models of retinal degeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801888/ https://www.ncbi.nlm.nih.gov/pubmed/31569695 http://dx.doi.org/10.3390/ijms20194807 |
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