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Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura

Nineteen polymorphic microsatellite loci were identified and developed for Natrix maura. Polymorphism was assessed for 120 individuals sampled across four sampling sites from the French Pyrenees Mountains. The number of alleles per locus ranged from 3 to 15, and expected heterozygosity per locus ran...

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Autores principales: Le Chevalier, Hugo, Marí‐Mena, Neus, Carro, Belén, Prunier, Jérôme G., Bossu, Coralie, Darnet, Elodie, Souchet, Jérémie, Guillaume, Olivier, Calvez, Olivier, Bertrand, Romain, Barthe, Laurent, Pottier, Gilles, Martínez‐Sylvestre, Albert, Verdaguer‐Foz, Isabel, Mossoll‐Torres, Marc, Trochet, Audrey, Aubret, Fabien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802021/
https://www.ncbi.nlm.nih.gov/pubmed/31641467
http://dx.doi.org/10.1002/ece3.5623
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author Le Chevalier, Hugo
Marí‐Mena, Neus
Carro, Belén
Prunier, Jérôme G.
Bossu, Coralie
Darnet, Elodie
Souchet, Jérémie
Guillaume, Olivier
Calvez, Olivier
Bertrand, Romain
Barthe, Laurent
Pottier, Gilles
Martínez‐Sylvestre, Albert
Verdaguer‐Foz, Isabel
Mossoll‐Torres, Marc
Trochet, Audrey
Aubret, Fabien
author_facet Le Chevalier, Hugo
Marí‐Mena, Neus
Carro, Belén
Prunier, Jérôme G.
Bossu, Coralie
Darnet, Elodie
Souchet, Jérémie
Guillaume, Olivier
Calvez, Olivier
Bertrand, Romain
Barthe, Laurent
Pottier, Gilles
Martínez‐Sylvestre, Albert
Verdaguer‐Foz, Isabel
Mossoll‐Torres, Marc
Trochet, Audrey
Aubret, Fabien
author_sort Le Chevalier, Hugo
collection PubMed
description Nineteen polymorphic microsatellite loci were identified and developed for Natrix maura. Polymorphism was assessed for 120 individuals sampled across four sampling sites from the French Pyrenees Mountains. The number of alleles per locus ranged from 3 to 15, and expected heterozygosity per locus ranged from 0.227 to 0.863. We tested for deviation from Hardy–Weinberg equilibrium and linkage disequilibrium and assessed the presence of null alleles for all loci, resulting in a selection of 14 high‐quality polymorphic markers. These markers will be extremely useful in identifying fine‐scale genetic structures and providing insight into conservation management plans of this species.
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spelling pubmed-68020212019-10-22 Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura Le Chevalier, Hugo Marí‐Mena, Neus Carro, Belén Prunier, Jérôme G. Bossu, Coralie Darnet, Elodie Souchet, Jérémie Guillaume, Olivier Calvez, Olivier Bertrand, Romain Barthe, Laurent Pottier, Gilles Martínez‐Sylvestre, Albert Verdaguer‐Foz, Isabel Mossoll‐Torres, Marc Trochet, Audrey Aubret, Fabien Ecol Evol Original Research Nineteen polymorphic microsatellite loci were identified and developed for Natrix maura. Polymorphism was assessed for 120 individuals sampled across four sampling sites from the French Pyrenees Mountains. The number of alleles per locus ranged from 3 to 15, and expected heterozygosity per locus ranged from 0.227 to 0.863. We tested for deviation from Hardy–Weinberg equilibrium and linkage disequilibrium and assessed the presence of null alleles for all loci, resulting in a selection of 14 high‐quality polymorphic markers. These markers will be extremely useful in identifying fine‐scale genetic structures and providing insight into conservation management plans of this species. John Wiley and Sons Inc. 2019-09-02 /pmc/articles/PMC6802021/ /pubmed/31641467 http://dx.doi.org/10.1002/ece3.5623 Text en © 2019 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Le Chevalier, Hugo
Marí‐Mena, Neus
Carro, Belén
Prunier, Jérôme G.
Bossu, Coralie
Darnet, Elodie
Souchet, Jérémie
Guillaume, Olivier
Calvez, Olivier
Bertrand, Romain
Barthe, Laurent
Pottier, Gilles
Martínez‐Sylvestre, Albert
Verdaguer‐Foz, Isabel
Mossoll‐Torres, Marc
Trochet, Audrey
Aubret, Fabien
Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura
title Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura
title_full Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura
title_fullStr Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura
title_full_unstemmed Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura
title_short Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura
title_sort isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake natrix maura
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802021/
https://www.ncbi.nlm.nih.gov/pubmed/31641467
http://dx.doi.org/10.1002/ece3.5623
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