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Light Regulation of Carotenoid Biosynthesis in the Peel of Mandarin and Sweet Orange Fruits

Carotenoids are the pigments responsible for the coloration of the peel and pulp of Citrus fruits. Light is one of the major environmental factors influencing coloration and carotenoid content and composition of fleshy fruits and therefore their commercial and nutritional quality. Agronomical observ...

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Autores principales: Lado, Joanna, Alós, Enriqueta, Manzi, Matías, Cronje, Paul J.R., Gómez-Cadenas, Aurelio, Rodrigo, María J., Zacarías, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803510/
https://www.ncbi.nlm.nih.gov/pubmed/31681382
http://dx.doi.org/10.3389/fpls.2019.01288
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author Lado, Joanna
Alós, Enriqueta
Manzi, Matías
Cronje, Paul J.R.
Gómez-Cadenas, Aurelio
Rodrigo, María J.
Zacarías, Lorenzo
author_facet Lado, Joanna
Alós, Enriqueta
Manzi, Matías
Cronje, Paul J.R.
Gómez-Cadenas, Aurelio
Rodrigo, María J.
Zacarías, Lorenzo
author_sort Lado, Joanna
collection PubMed
description Carotenoids are the pigments responsible for the coloration of the peel and pulp of Citrus fruits. Light is one of the major environmental factors influencing coloration and carotenoid content and composition of fleshy fruits and therefore their commercial and nutritional quality. Agronomical observations indicate that citrus fruits exposed to sunlight develop a brighter peel coloration than shaded fruit inside the tree canopy. In the present study, the effect of light deprivation on carotenoid profile, and in the expression of genes of carotenoid metabolism and their precursors have been analyzed in fruits of Clemenules mandarin (Citrus clementine) and Navelina orange (Citrus sinensis). Fruit shading accelerated peel degreening, chlorophyll degradation, and reduced chloroplastic-type carotenoids. Time-course shading experiments revealed that the stage of fruit ripening appears to be determinant for the effect of darkness in carotenoid biosynthesis. Fruit shading produced a down-regulation of the expression of key carotenoids biosynthetic genes (PSY, PDS, ZDS1, LCY2a, LCY2b, and CHX). However, expression of MEP pathway genes (DXS, HDR1, and GGPPS1) and the carotenoid cleavage dioxygenase, CCD4b1, responsible of the formation of the apocarotenoid β-citraurin, were not substantially affected by dark-grown conditions. The content of abscisic acid (ABA), an end product of the carotenoid pathway, was not affected by the light regime, suggesting that effect of shading on the precursor’s pool is not sufficient to impair ABA synthesis. A moderate increase in total carotenoid and in the expression of biosynthetic genes was observed in mature dark-grown mandarin and orange fruits. Collectively, results suggest that light stimulates carotenoid biosynthesis in the peel of citrus fruits but a light-independent regulation may also operate.
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spelling pubmed-68035102019-11-03 Light Regulation of Carotenoid Biosynthesis in the Peel of Mandarin and Sweet Orange Fruits Lado, Joanna Alós, Enriqueta Manzi, Matías Cronje, Paul J.R. Gómez-Cadenas, Aurelio Rodrigo, María J. Zacarías, Lorenzo Front Plant Sci Plant Science Carotenoids are the pigments responsible for the coloration of the peel and pulp of Citrus fruits. Light is one of the major environmental factors influencing coloration and carotenoid content and composition of fleshy fruits and therefore their commercial and nutritional quality. Agronomical observations indicate that citrus fruits exposed to sunlight develop a brighter peel coloration than shaded fruit inside the tree canopy. In the present study, the effect of light deprivation on carotenoid profile, and in the expression of genes of carotenoid metabolism and their precursors have been analyzed in fruits of Clemenules mandarin (Citrus clementine) and Navelina orange (Citrus sinensis). Fruit shading accelerated peel degreening, chlorophyll degradation, and reduced chloroplastic-type carotenoids. Time-course shading experiments revealed that the stage of fruit ripening appears to be determinant for the effect of darkness in carotenoid biosynthesis. Fruit shading produced a down-regulation of the expression of key carotenoids biosynthetic genes (PSY, PDS, ZDS1, LCY2a, LCY2b, and CHX). However, expression of MEP pathway genes (DXS, HDR1, and GGPPS1) and the carotenoid cleavage dioxygenase, CCD4b1, responsible of the formation of the apocarotenoid β-citraurin, were not substantially affected by dark-grown conditions. The content of abscisic acid (ABA), an end product of the carotenoid pathway, was not affected by the light regime, suggesting that effect of shading on the precursor’s pool is not sufficient to impair ABA synthesis. A moderate increase in total carotenoid and in the expression of biosynthetic genes was observed in mature dark-grown mandarin and orange fruits. Collectively, results suggest that light stimulates carotenoid biosynthesis in the peel of citrus fruits but a light-independent regulation may also operate. Frontiers Media S.A. 2019-10-15 /pmc/articles/PMC6803510/ /pubmed/31681382 http://dx.doi.org/10.3389/fpls.2019.01288 Text en Copyright © 2019 Lado, Alós, Manzi, Cronje, Gómez-Cadenas, Rodrigo and Zacarías http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lado, Joanna
Alós, Enriqueta
Manzi, Matías
Cronje, Paul J.R.
Gómez-Cadenas, Aurelio
Rodrigo, María J.
Zacarías, Lorenzo
Light Regulation of Carotenoid Biosynthesis in the Peel of Mandarin and Sweet Orange Fruits
title Light Regulation of Carotenoid Biosynthesis in the Peel of Mandarin and Sweet Orange Fruits
title_full Light Regulation of Carotenoid Biosynthesis in the Peel of Mandarin and Sweet Orange Fruits
title_fullStr Light Regulation of Carotenoid Biosynthesis in the Peel of Mandarin and Sweet Orange Fruits
title_full_unstemmed Light Regulation of Carotenoid Biosynthesis in the Peel of Mandarin and Sweet Orange Fruits
title_short Light Regulation of Carotenoid Biosynthesis in the Peel of Mandarin and Sweet Orange Fruits
title_sort light regulation of carotenoid biosynthesis in the peel of mandarin and sweet orange fruits
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803510/
https://www.ncbi.nlm.nih.gov/pubmed/31681382
http://dx.doi.org/10.3389/fpls.2019.01288
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