Transcription Analysis of the Stress and Immune Response Genes to Temperature Stress in Ostrinia furnacalis

Ostrinia furnacalis is one of the most important pests on maize. O. furnacalis larvae are frequently exposed to the temperature challenges such as high temperature in summer and cold temperature in winter in the natural environment. High and low temperature stress, like any abiotic stress, impairs t...

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Autores principales: Chen, Kangkang, Tang, Tai, Song, Qisheng, Wang, Zhenying, He, Kanglai, Liu, Xu, Song, Jiahui, Wang, Libao, Yang, Yizhong, Feng, Congjing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803539/
https://www.ncbi.nlm.nih.gov/pubmed/31681003
http://dx.doi.org/10.3389/fphys.2019.01289
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author Chen, Kangkang
Tang, Tai
Song, Qisheng
Wang, Zhenying
He, Kanglai
Liu, Xu
Song, Jiahui
Wang, Libao
Yang, Yizhong
Feng, Congjing
author_facet Chen, Kangkang
Tang, Tai
Song, Qisheng
Wang, Zhenying
He, Kanglai
Liu, Xu
Song, Jiahui
Wang, Libao
Yang, Yizhong
Feng, Congjing
author_sort Chen, Kangkang
collection PubMed
description Ostrinia furnacalis is one of the most important pests on maize. O. furnacalis larvae are frequently exposed to the temperature challenges such as high temperature in summer and cold temperature in winter in the natural environment. High and low temperature stress, like any abiotic stress, impairs the physiology and development of insects. Up to now, there is limited information about gene regulation and signaling pathways related to the high and cold stress response in O. furnacalis. High-throughput sequencing of transcriptome provides a new approach for detecting stress and immune response genes under high and low temperature stresses in O. furnacalis. In the present study, O. furnacalis larvae were treated with the temperature at 8 and 40°C, and the responses of O. furnacalis larvae to the temperature stress were investigated through RNA-sequencing and further confirmation. The results showed that immune responses were up-regulated in larvae by the cold stress at 8°C while some stress response genes, such as HSP family, GST-2, Bax inhibitor and P450, were significantly increased at 40°C. Furthermore, quantitative real time polymerase chain reaction were performed to quantify the expression levels of immune related genes, such as PGRP-LB, antimicrobial peptides, lysozyme, serine protease and stress response genes such as small HSPs and HSP90, and the expression levels of these genes were similar to the RNA-seq results. In addition, the iron storage protein Ferritin was found to be involved in the response to temperature stress, and the changes of total iron concentration in the hemolymph were, in general, consistent with the expression levels of Ferritin. Taken together, our results suggested that the stress response genes were involved in the defense against the heat stress at 40°C, and the immune responses triggered by cold stress might provide protection for larvae from cold stress at 8°C. More interestingly, our results showed that during the responses to temperature stress, the total iron concentration in hemolymph regulated by Ferritin increased, which might help O. furnacalis in surviving the low and high temperature stress.
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spelling pubmed-68035392019-11-03 Transcription Analysis of the Stress and Immune Response Genes to Temperature Stress in Ostrinia furnacalis Chen, Kangkang Tang, Tai Song, Qisheng Wang, Zhenying He, Kanglai Liu, Xu Song, Jiahui Wang, Libao Yang, Yizhong Feng, Congjing Front Physiol Physiology Ostrinia furnacalis is one of the most important pests on maize. O. furnacalis larvae are frequently exposed to the temperature challenges such as high temperature in summer and cold temperature in winter in the natural environment. High and low temperature stress, like any abiotic stress, impairs the physiology and development of insects. Up to now, there is limited information about gene regulation and signaling pathways related to the high and cold stress response in O. furnacalis. High-throughput sequencing of transcriptome provides a new approach for detecting stress and immune response genes under high and low temperature stresses in O. furnacalis. In the present study, O. furnacalis larvae were treated with the temperature at 8 and 40°C, and the responses of O. furnacalis larvae to the temperature stress were investigated through RNA-sequencing and further confirmation. The results showed that immune responses were up-regulated in larvae by the cold stress at 8°C while some stress response genes, such as HSP family, GST-2, Bax inhibitor and P450, were significantly increased at 40°C. Furthermore, quantitative real time polymerase chain reaction were performed to quantify the expression levels of immune related genes, such as PGRP-LB, antimicrobial peptides, lysozyme, serine protease and stress response genes such as small HSPs and HSP90, and the expression levels of these genes were similar to the RNA-seq results. In addition, the iron storage protein Ferritin was found to be involved in the response to temperature stress, and the changes of total iron concentration in the hemolymph were, in general, consistent with the expression levels of Ferritin. Taken together, our results suggested that the stress response genes were involved in the defense against the heat stress at 40°C, and the immune responses triggered by cold stress might provide protection for larvae from cold stress at 8°C. More interestingly, our results showed that during the responses to temperature stress, the total iron concentration in hemolymph regulated by Ferritin increased, which might help O. furnacalis in surviving the low and high temperature stress. Frontiers Media S.A. 2019-10-15 /pmc/articles/PMC6803539/ /pubmed/31681003 http://dx.doi.org/10.3389/fphys.2019.01289 Text en Copyright © 2019 Chen, Tang, Song, Wang, He, Liu, Song, Wang, Yang and Feng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Chen, Kangkang
Tang, Tai
Song, Qisheng
Wang, Zhenying
He, Kanglai
Liu, Xu
Song, Jiahui
Wang, Libao
Yang, Yizhong
Feng, Congjing
Transcription Analysis of the Stress and Immune Response Genes to Temperature Stress in Ostrinia furnacalis
title Transcription Analysis of the Stress and Immune Response Genes to Temperature Stress in Ostrinia furnacalis
title_full Transcription Analysis of the Stress and Immune Response Genes to Temperature Stress in Ostrinia furnacalis
title_fullStr Transcription Analysis of the Stress and Immune Response Genes to Temperature Stress in Ostrinia furnacalis
title_full_unstemmed Transcription Analysis of the Stress and Immune Response Genes to Temperature Stress in Ostrinia furnacalis
title_short Transcription Analysis of the Stress and Immune Response Genes to Temperature Stress in Ostrinia furnacalis
title_sort transcription analysis of the stress and immune response genes to temperature stress in ostrinia furnacalis
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803539/
https://www.ncbi.nlm.nih.gov/pubmed/31681003
http://dx.doi.org/10.3389/fphys.2019.01289
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