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Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols

Apples are a nutritious food source with significant amounts of polyphenols that contribute to human health and wellbeing, primarily as dietary antioxidants. Although numerous pre- and post-harvest factors can affect the composition of polyphenols in apples, genetics is presumed to play a major role...

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Autores principales: McClure, Kendra A., Gong, YuiHui, Song, Jun, Vinqvist-Tymchuk, Melinda, Campbell Palmer, Leslie, Fan, Lihua, Burgher-MacLellan, Karen, Zhang, ZhaoQi, Celton, Jean-Marc, Forney, Charles F., Migicovsky, Zoë, Myles, Sean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804656/
https://www.ncbi.nlm.nih.gov/pubmed/31645962
http://dx.doi.org/10.1038/s41438-019-0190-y
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author McClure, Kendra A.
Gong, YuiHui
Song, Jun
Vinqvist-Tymchuk, Melinda
Campbell Palmer, Leslie
Fan, Lihua
Burgher-MacLellan, Karen
Zhang, ZhaoQi
Celton, Jean-Marc
Forney, Charles F.
Migicovsky, Zoë
Myles, Sean
author_facet McClure, Kendra A.
Gong, YuiHui
Song, Jun
Vinqvist-Tymchuk, Melinda
Campbell Palmer, Leslie
Fan, Lihua
Burgher-MacLellan, Karen
Zhang, ZhaoQi
Celton, Jean-Marc
Forney, Charles F.
Migicovsky, Zoë
Myles, Sean
author_sort McClure, Kendra A.
collection PubMed
description Apples are a nutritious food source with significant amounts of polyphenols that contribute to human health and wellbeing, primarily as dietary antioxidants. Although numerous pre- and post-harvest factors can affect the composition of polyphenols in apples, genetics is presumed to play a major role because polyphenol concentration varies dramatically among apple cultivars. Here we investigated the genetic architecture of apple polyphenols by combining high performance liquid chromatography (HPLC) data with ~100,000 single nucleotide polymorphisms (SNPs) from two diverse apple populations. We found that polyphenols can vary in concentration by up to two orders of magnitude across cultivars, and that this dramatic variation was often predictable using genetic markers and frequently controlled by a small number of large effect genetic loci. Using GWAS, we identified candidate genes for the production of quercitrin, epicatechin, catechin, chlorogenic acid, 4-O-caffeoylquinic acid and procyanidins B1, B2, and C1. Our observation that a relatively simple genetic architecture underlies the dramatic variation of key polyphenols in apples suggests that breeders may be able to improve the nutritional value of apples through marker-assisted breeding or gene editing.
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spelling pubmed-68046562019-10-23 Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols McClure, Kendra A. Gong, YuiHui Song, Jun Vinqvist-Tymchuk, Melinda Campbell Palmer, Leslie Fan, Lihua Burgher-MacLellan, Karen Zhang, ZhaoQi Celton, Jean-Marc Forney, Charles F. Migicovsky, Zoë Myles, Sean Hortic Res Article Apples are a nutritious food source with significant amounts of polyphenols that contribute to human health and wellbeing, primarily as dietary antioxidants. Although numerous pre- and post-harvest factors can affect the composition of polyphenols in apples, genetics is presumed to play a major role because polyphenol concentration varies dramatically among apple cultivars. Here we investigated the genetic architecture of apple polyphenols by combining high performance liquid chromatography (HPLC) data with ~100,000 single nucleotide polymorphisms (SNPs) from two diverse apple populations. We found that polyphenols can vary in concentration by up to two orders of magnitude across cultivars, and that this dramatic variation was often predictable using genetic markers and frequently controlled by a small number of large effect genetic loci. Using GWAS, we identified candidate genes for the production of quercitrin, epicatechin, catechin, chlorogenic acid, 4-O-caffeoylquinic acid and procyanidins B1, B2, and C1. Our observation that a relatively simple genetic architecture underlies the dramatic variation of key polyphenols in apples suggests that breeders may be able to improve the nutritional value of apples through marker-assisted breeding or gene editing. Nature Publishing Group UK 2019-09-07 /pmc/articles/PMC6804656/ /pubmed/31645962 http://dx.doi.org/10.1038/s41438-019-0190-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
McClure, Kendra A.
Gong, YuiHui
Song, Jun
Vinqvist-Tymchuk, Melinda
Campbell Palmer, Leslie
Fan, Lihua
Burgher-MacLellan, Karen
Zhang, ZhaoQi
Celton, Jean-Marc
Forney, Charles F.
Migicovsky, Zoë
Myles, Sean
Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols
title Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols
title_full Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols
title_fullStr Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols
title_full_unstemmed Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols
title_short Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols
title_sort genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804656/
https://www.ncbi.nlm.nih.gov/pubmed/31645962
http://dx.doi.org/10.1038/s41438-019-0190-y
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