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Universal Template-Assisted, Cloning-free Method for the Generation of Small RNA-Expressing Dumbbell-Shaped DNA Vectors

Dumbbell-shaped DNA minimal vectors represent genetic vectors solely composed of the gene expression cassette of interest and terminal closing loop structures. Dumbbell vectors for small hairpin RNA or microRNA expression are extremely small-sized, which is advantageous with regard to cellular deliv...

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Detalles Bibliográficos
Autores principales: Cyrill, Samantha Leeanne, Ghosh, Avantika, Loh, Pei She, Tan, Genim Siu Xian, Patzel, Volker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6807298/
https://www.ncbi.nlm.nih.gov/pubmed/31660417
http://dx.doi.org/10.1016/j.omtm.2019.08.008