On-slide tissue digestion for mass spectrometry based glycomic and proteomic profiling

We describe a protocol for glycomic and proteomic profiling that uses serial enzyme digestions from the surface of fresh frozen or fixed tissue slides. The abundances of the extracted glycans and peptides are determined using liquid chromatography-mass spectrometry. In a typical experiment, our meth...

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Detalles Bibliográficos
Autores principales: Raghunathan, Rekha, Sethi, Manveen K., Zaia, Joseph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6807300/
https://www.ncbi.nlm.nih.gov/pubmed/31660297
http://dx.doi.org/10.1016/j.mex.2019.09.029

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6807300/
https://www.ncbi.nlm.nih.gov/pubmed/31660297
http://dx.doi.org/10.1016/j.mex.2019.09.029

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