Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have been harnessed as powerful genome editing tools in diverse organisms. However, the off-target effects and the protospacer adjacent motif (PAM) compatibility restrict the therapeutic applications of these systems. Rec...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Weizhong, Zhang, Hongyuan, Zhang, Yifei, Wang, Yu, Gan, Jianhua, Ji, Quanjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6808508/
https://www.ncbi.nlm.nih.gov/pubmed/31603896
http://dx.doi.org/10.1371/journal.pbio.3000496

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6808508/
https://www.ncbi.nlm.nih.gov/pubmed/31603896
http://dx.doi.org/10.1371/journal.pbio.3000496

Ejemplares similares