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Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have been harnessed as powerful genome editing tools in diverse organisms. However, the off-target effects and the protospacer adjacent motif (PAM) compatibility restrict the therapeutic applications of these systems. Rec...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6808508/ https://www.ncbi.nlm.nih.gov/pubmed/31603896 http://dx.doi.org/10.1371/journal.pbio.3000496 |