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660. Extraction-Free 16S Ribosomal RNA (rRNA) Gene Amplification and Sequencing from Resected Cardiac Implantable Electronic Device (CIED) Sonicate Fluid

BACKGROUND: We recently demonstrated that 16S rRNA PCR/sequencing performed on biofilms dislodged from extracted CIEDs into a salt solution—referred to as “sonicate fluid’ (SF)—may be used to detect pathogens in culture-negative CIED infection (Clinical Infectious Diseases, ciz266, doi:10.1093/cid/c...

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Detalles Bibliográficos
Autores principales: Esquer Garrigos, Zerelda, Sohail, Muhammad R, Greenwood-Quaintance, Kerryl, Cunningham, Scott A, Vijayvargiya, Prakhar, Patel, Robin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6811131/
http://dx.doi.org/10.1093/ofid/ofz360.728