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660. Extraction-Free 16S Ribosomal RNA (rRNA) Gene Amplification and Sequencing from Resected Cardiac Implantable Electronic Device (CIED) Sonicate Fluid
BACKGROUND: We recently demonstrated that 16S rRNA PCR/sequencing performed on biofilms dislodged from extracted CIEDs into a salt solution—referred to as “sonicate fluid’ (SF)—may be used to detect pathogens in culture-negative CIED infection (Clinical Infectious Diseases, ciz266, doi:10.1093/cid/c...
Autores principales: | Esquer Garrigos, Zerelda, Sohail, Muhammad R, Greenwood-Quaintance, Kerryl, Cunningham, Scott A, Vijayvargiya, Prakhar, Patel, Robin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6811131/ http://dx.doi.org/10.1093/ofid/ofz360.728 |
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