Cargando…

CRISPR-Cas12a Nucleases Bind Flexible DNA Duplexes without RNA/DNA Complementarity

[Image: see text] Cas12a (also known as “Cpf1”) is a class 2 type V-A CRISPR-associated nuclease that can cleave double-stranded DNA at specific sites. The Cas12a effector enzyme comprises a single protein and a CRISPR-encoded small RNA (crRNA) and has been used for genome editing and manipulation....

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Wei, Singh, Jaideep, Allen, Aleique, Li, Yue, Kathiresan, Venkatesan, Qureshi, Omair, Tangprasertchai, Narin, Zhang, Xiaojun, Parameshwaran, Hari Priya, Rajan, Rakhi, Qin, Peter Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6811856/
https://www.ncbi.nlm.nih.gov/pubmed/31656887
http://dx.doi.org/10.1021/acsomega.9b01469
Descripción
Sumario:[Image: see text] Cas12a (also known as “Cpf1”) is a class 2 type V-A CRISPR-associated nuclease that can cleave double-stranded DNA at specific sites. The Cas12a effector enzyme comprises a single protein and a CRISPR-encoded small RNA (crRNA) and has been used for genome editing and manipulation. Work reported here examined in vitro interactions between the Cas12a effector enzyme and DNA duplexes with varying states of base-pairing between the two strands. The data revealed that in the absence of complementarity between the crRNA guide and the DNA target-strand, Cas12a binds duplexes with unpaired segments. These off-target duplexes were bound at the Cas12a site responsible for RNA-guided double-stranded DNA binding but were not cleaved due to the lack of RNA/DNA hybrid formation. Such promiscuous binding was attributed to increased DNA flexibility induced by the unpaired segment present next to the protospacer-adjacent-motif. The results suggest that target discrimination of Cas12a can be influenced by flexibility of the DNA. As such, in addition to the linear sequence, flexibility and other physical properties of the DNA should be considered in Cas12a-based genome engineering applications.