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A new method for extracting DNA from the grape berry surface, beginning in the vineyard
Isolating DNA from microbes on the surface of a grape berry is a challenge due to their adhesion to the thick berry skin and cuticle, making studies of the grape microbiome challenging. We developed a field-to-lab DNA extraction procedure that starts in the vineyard, disrupts the grape berry surface...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812244/ https://www.ncbi.nlm.nih.gov/pubmed/31667416 http://dx.doi.org/10.1016/j.heliyon.2019.e02597 |
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author | Hall, Megan E. Cadle-Davidson, Lance Fang, Zhiwei Wilcox, Wayne F. |
author_facet | Hall, Megan E. Cadle-Davidson, Lance Fang, Zhiwei Wilcox, Wayne F. |
author_sort | Hall, Megan E. |
collection | PubMed |
description | Isolating DNA from microbes on the surface of a grape berry is a challenge due to their adhesion to the thick berry skin and cuticle, making studies of the grape microbiome challenging. We developed a field-to-lab DNA extraction procedure that starts in the vineyard, disrupts the grape berry surface while en route to the lab through agitation, and efficiently extracts microbial DNA from the surface of the grape. It is cost effective and utilizes commonly available laboratory chemicals with low toxicity (Table 1). This protocol allows researchers to extract DNA from the grape berry surface in the field, therefore undergoing minimal manipulation of those microbiomes before DNA extraction. |
format | Online Article Text |
id | pubmed-6812244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-68122442019-10-30 A new method for extracting DNA from the grape berry surface, beginning in the vineyard Hall, Megan E. Cadle-Davidson, Lance Fang, Zhiwei Wilcox, Wayne F. Heliyon Article Isolating DNA from microbes on the surface of a grape berry is a challenge due to their adhesion to the thick berry skin and cuticle, making studies of the grape microbiome challenging. We developed a field-to-lab DNA extraction procedure that starts in the vineyard, disrupts the grape berry surface while en route to the lab through agitation, and efficiently extracts microbial DNA from the surface of the grape. It is cost effective and utilizes commonly available laboratory chemicals with low toxicity (Table 1). This protocol allows researchers to extract DNA from the grape berry surface in the field, therefore undergoing minimal manipulation of those microbiomes before DNA extraction. Elsevier 2019-10-05 /pmc/articles/PMC6812244/ /pubmed/31667416 http://dx.doi.org/10.1016/j.heliyon.2019.e02597 Text en © 2019 Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Hall, Megan E. Cadle-Davidson, Lance Fang, Zhiwei Wilcox, Wayne F. A new method for extracting DNA from the grape berry surface, beginning in the vineyard |
title | A new method for extracting DNA from the grape berry surface, beginning in the vineyard |
title_full | A new method for extracting DNA from the grape berry surface, beginning in the vineyard |
title_fullStr | A new method for extracting DNA from the grape berry surface, beginning in the vineyard |
title_full_unstemmed | A new method for extracting DNA from the grape berry surface, beginning in the vineyard |
title_short | A new method for extracting DNA from the grape berry surface, beginning in the vineyard |
title_sort | new method for extracting dna from the grape berry surface, beginning in the vineyard |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812244/ https://www.ncbi.nlm.nih.gov/pubmed/31667416 http://dx.doi.org/10.1016/j.heliyon.2019.e02597 |
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