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Decapping Scavenger Enzyme Activity toward N2-Substituted 5′ End mRNA Cap Analogues

[Image: see text] mRNA degradation is a key mechanism of gene expression regulation. In the 3′ → 5′ decay pathway, mRNA is degraded by the exosome complex and the resulting cap dinucleotide or short-capped oligonucleotide is hydrolyzed mainly by a decapping scavenger enzyme (DcpS)—a member of the hi...

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Autores principales: Pietrow, Paulina, Ferenc-Mrozek, Aleksandra, Piecyk, Karolina, Bojarska, Elzbieta, Darzynkiewicz, Edward, Jankowska-Anyszka, Marzena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812366/
https://www.ncbi.nlm.nih.gov/pubmed/31656932
http://dx.doi.org/10.1021/acsomega.9b02715
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author Pietrow, Paulina
Ferenc-Mrozek, Aleksandra
Piecyk, Karolina
Bojarska, Elzbieta
Darzynkiewicz, Edward
Jankowska-Anyszka, Marzena
author_facet Pietrow, Paulina
Ferenc-Mrozek, Aleksandra
Piecyk, Karolina
Bojarska, Elzbieta
Darzynkiewicz, Edward
Jankowska-Anyszka, Marzena
author_sort Pietrow, Paulina
collection PubMed
description [Image: see text] mRNA degradation is a key mechanism of gene expression regulation. In the 3′ → 5′ decay pathway, mRNA is degraded by the exosome complex and the resulting cap dinucleotide or short-capped oligonucleotide is hydrolyzed mainly by a decapping scavenger enzyme (DcpS)—a member of the histidine triad family. The decapping mechanism is similar for DcpS from different species; however, their respective substrate specificities differ. In this paper, we describe experiments exploring DcpS activity from human (hDcps), Caenorhabditis elegans (CeDcpS), and Ascaris suum (AsDcpS) toward dinucleotide cap analogues modified at the N2 position of 7-methylguanosine. Various alkyl substituents were tested, and cap analogues with a longer than three-carbon chain were nonhydrolyzable by hDcpS and CeDcpS. Resistance of the modified cap analogues to hDcpS and CeDcpS may be associated with their weaker binding with enzymes.
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spelling pubmed-68123662019-10-25 Decapping Scavenger Enzyme Activity toward N2-Substituted 5′ End mRNA Cap Analogues Pietrow, Paulina Ferenc-Mrozek, Aleksandra Piecyk, Karolina Bojarska, Elzbieta Darzynkiewicz, Edward Jankowska-Anyszka, Marzena ACS Omega [Image: see text] mRNA degradation is a key mechanism of gene expression regulation. In the 3′ → 5′ decay pathway, mRNA is degraded by the exosome complex and the resulting cap dinucleotide or short-capped oligonucleotide is hydrolyzed mainly by a decapping scavenger enzyme (DcpS)—a member of the histidine triad family. The decapping mechanism is similar for DcpS from different species; however, their respective substrate specificities differ. In this paper, we describe experiments exploring DcpS activity from human (hDcps), Caenorhabditis elegans (CeDcpS), and Ascaris suum (AsDcpS) toward dinucleotide cap analogues modified at the N2 position of 7-methylguanosine. Various alkyl substituents were tested, and cap analogues with a longer than three-carbon chain were nonhydrolyzable by hDcpS and CeDcpS. Resistance of the modified cap analogues to hDcpS and CeDcpS may be associated with their weaker binding with enzymes. American Chemical Society 2019-10-09 /pmc/articles/PMC6812366/ /pubmed/31656932 http://dx.doi.org/10.1021/acsomega.9b02715 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Pietrow, Paulina
Ferenc-Mrozek, Aleksandra
Piecyk, Karolina
Bojarska, Elzbieta
Darzynkiewicz, Edward
Jankowska-Anyszka, Marzena
Decapping Scavenger Enzyme Activity toward N2-Substituted 5′ End mRNA Cap Analogues
title Decapping Scavenger Enzyme Activity toward N2-Substituted 5′ End mRNA Cap Analogues
title_full Decapping Scavenger Enzyme Activity toward N2-Substituted 5′ End mRNA Cap Analogues
title_fullStr Decapping Scavenger Enzyme Activity toward N2-Substituted 5′ End mRNA Cap Analogues
title_full_unstemmed Decapping Scavenger Enzyme Activity toward N2-Substituted 5′ End mRNA Cap Analogues
title_short Decapping Scavenger Enzyme Activity toward N2-Substituted 5′ End mRNA Cap Analogues
title_sort decapping scavenger enzyme activity toward n2-substituted 5′ end mrna cap analogues
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812366/
https://www.ncbi.nlm.nih.gov/pubmed/31656932
http://dx.doi.org/10.1021/acsomega.9b02715
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